Tocopherols and tocotrienols are chemical compounds insusceptible to the ionization process under atmospheric pressure conditions. Therefore, the selection of the optimal ion source settings for their quantification requires special attention. The aim of this study was to analyse the influence of the APCI source parameters on the response of tocochromanols and two related compounds.
Standard solutions of target compounds were injected on the HPLC-APCI-MS/MS system separately and analysed in 30 randomly selected ion source settings. The obtained responses were modelled by multivariate linear regression with least absolute shrinkage and selection operator. The developed models were used for choosing best APCI conditions.
Multivariate linear models were built for eight tocochromanols, trolox and BHT. The APCI settings derived from the models did not increase the peak areas obtained for T and T3 during ionization process. Ionization conditions based on models for trolox and BHT improved analytical responses for 12-36% and 4-32%, respectively. The application of the ion source settings optimal for trolox and BHT to tocochromanols did not result in better analytical responses.
The ionization pattern of tocochromanols in APCI source is problematic and should be further investigated. Modelling methodology for response improvement presented in this study can be applied in similar studies.
Tocopherol is one of the 2 members of the vitamin E family. The other member is known as tocotrienol. Vitamin E incorporates 8 different compounds. These include 4 tocopherols (alpha, beta, gamma and delta) and 4 tocotrienols (alpha, beta, gamma and delta). Both of them require fat in the diet in order to be absorbed and distributed within the body. Although it may seem like the two members are similar, various differences exist between them as outlined below.
Suffering from stress-induced gastritis? Palm vitamin E may be the solution. Studies on the use of this vitamin in rats have shown that it reduces stress-induced gastritis. The rats, which were pre-treated with palm vitamin E, had gastritis induced in them following the stress of being immersed in water.
They are a source of energy and nutrients for our bodies, as well as antioxidants that protect our cells against the effects of free radicals and help to reduce inflammation. These are the good oils that should form a small but essential part of our daily diet. While there are many types of oils out there, an excellent one in our very own backyard is palm oil, which contains a high level of vitamin E.
Hair fall is a pain. Blame it on our lifestyles, pollution or rampant stress but losing hair is one of the greatest beauty concerns that most women are dealing with today. There are hundreds of miracle products that promise quick hair growth and reducing hair fall but how many can you really vouch have worked for you? That’s probably because most of these products miss one important ingredient that actually stimulates hair growth and can give you the voluminous movie siren hair you have always wanted. We are talking about vitamin E, the most effective and essential ingredient for hair growth.
Higher blood levels of Vitamin E may be linked to a lower risk of gallstone disease, finds a recent study published in Nutrients.
In the 1970s, researchers attempted to discover the symptoms of a vitamin E deficiency by providing subjects (read: graduate students) with a diet completely devoid of vitamin E. After a year on this diet, the subjects showed no signs of deficiency. The researchers concluded that vitamin E was vitamin in search of a deficiency syndrome (such as C and scurvy or D and rickets). Later, researchers found that many nutrient deficiencies do not show up as a classical deficiency syndrome, but rather surface years later as heart disease, cancer, or some other catastrophic disease.
A new study revealed that vitamin E — in particular tocotrienol — could improve the bone density of postmenopausal women. The study was carried out by a group of scientists from the Texas Tech University Health Sciences Center and the Georgia State University who assessed the benefit of vitamin E to bone health.
“This study showed that supplementation of tocotrienols, mainly delta-tocotrienols, suppressed bone [bone remodeling regulators],” the researchers wrote in the report. “Such osteoprotective tocotrienol’s effects may be, in part, mediated by an inhibition of oxidative stress.”