Tocopherols and tocotrienols are chemical compounds insusceptible to the ionization process under atmospheric pressure conditions. Therefore, the selection of the optimal ion source settings for their quantification requires special attention. The aim of this study was to analyse the influence of the APCI source parameters on the response of tocochromanols and two related compounds.
Standard solutions of target compounds were injected on the HPLC-APCI-MS/MS system separately and analysed in 30 randomly selected ion source settings. The obtained responses were modelled by multivariate linear regression with least absolute shrinkage and selection operator. The developed models were used for choosing best APCI conditions.
Multivariate linear models were built for eight tocochromanols, trolox and BHT. The APCI settings derived from the models did not increase the peak areas obtained for T and T3 during ionization process. Ionization conditions based on models for trolox and BHT improved analytical responses for 12-36% and 4-32%, respectively. The application of the ion source settings optimal for trolox and BHT to tocochromanols did not result in better analytical responses.
The ionization pattern of tocochromanols in APCI source is problematic and should be further investigated. Modelling methodology for response improvement presented in this study can be applied in similar studies.
According to a Smart Publications research report, “red palm oil has a higher bioavailability of antioxidant nutrients (proportion of nutrients that are usable by the body) than other vegetable sources and is a particularly important dietary oil for people who are not taking an excellent vitamin E supplement, with tocopherols and tocotrienols, and full-spectrum carotenoid nutritional supplement. It is considered the richest natural source of carotenoids with concentrations of 700- 1000 ppm. That’s 30 times more than is contained in carrots!”
As the days grow longer, the sun hotter and the hours spent outside more plentiful, it’s important to know which supplements to take to keep your body functioning in the heat. A study published by the U.S. National Library of Medicine found that vitamin C and vitamin E supplements can help protect skin against harmful ultraviolet radiation. This means less DNA damage, fewer sunburns and decreased risk of skin cancer. However, to reap the shielding benefits of these vitamins, these supplements must be taken together. Only taking vitamin C can increase immune function, and vitamin E on its own is an antioxidant that takes out potentially damaging free radicals. But if you want to enjoy the anti-ultraviolet effects of these supplements, it’s critical to take them both.
A form of Vitamin E may help protect against high levels of radiation exposure. Studies show that a potent form of Vitamin E called gamma tocotrienols may counteract the harmful effects of radiation. “It is something we have been working on for about five years now,” says Dr. Martin Hauer-Jensen, director of radiation health at UAMS.
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